PDOX for Brain Metastasis Studies

LIDE has established brain metastatic patient-derived orthotropic xenografts (PDOXs) models, including metastasis from breast and gastric cancers.

Model ID Source CRCs Luciferase labeling In vivo evaluation
GL261 Murine glioblastoma cancer cell line N/A N/A Completed
U87-MG Human glioblastoma cancer cell line N/A Completed Completed
U251 Human glioblastoma cancer cell line N/A Completed TBD
LD1-0020-200615 Human glioblastoma PDX Completed Completed Completed
LD1-2009-362263 Human breast cancer brain metastasis Completed Completed Completed
LD1-2009-362541 Human breast cancer brain metastasis Completed Completed Completed
LD1-2017-362457 Human gastric cancer brain metastasis TBD TBD TBD
LD1-0011-362257 Human HCC brain metastatic PDX TBD TBD TBD
LD1-2059-362445 Human NSCLC brain metastatic PDX TBD TBD TBD


Methodology:

  1. This novel method starts with using samples from patients with primary tumor that metastasized to the brain (tissue, cerebrospinal fluid, etc.).
  2. Cell populations are generated using conditionally reprogrammed cells, which are further labeled with luciferase.
  3. These cells are inoculated into mice ventricles via a stereotaxic apparatus.
  4. Survival of mice is recorded and intensity of fluorescence can be tracked in real time using In Vivo Imaging System (IVIS).

Fig. Schematic of PDOX process for brain tumors

 

 

 

 

 

 

 

 

 

 

 


Fig. Schematic of PDOX process for brain tumors